Estimation of relative mRNA content by filter hybridization to a polyuridylic probe.
نویسندگان
چکیده
1.Battaglia, M., P. Pedrazzoli, B. Palermo, A. Lanza, F. Bertolini, N. Gibelli, G.A. Da Prada, A. Zambelli et al. 1998. Epithelial tumour cell detection and the unsolved problems of nested RT-PCR: a new sensitive one step method without false positive results. Bone Marrow Transplant 22:693-698. 2.Collins, C., J.M. Rommens, D. Kowbel, T. Godfrey, M. Tanner, S.I. Hwang, D. Polikoff, G. Nonet et al. 1998. Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma. Proc. Natl. Acad. Sci. USA 95:8703-8708. 3.Gibson, U.E., C.A. Heid and P.M. Williams. 1996. A novel method for real time quantitative RT-PCR. Genome Res. 6:995-1001. 4.Heid, C.A., J. Stevens, K.J. Livak and P.M. Williams. 1996. Real time quantitative PCR. Genome Res. 6:986-994. 5.PE Applied Biosystems User Bulletin #2. 1997. Relative quantitation of gene expression. Applied Biosystems, Foster City, CA.
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ورودعنوان ژورنال:
- BioTechniques
دوره 29 4 شماره
صفحات -
تاریخ انتشار 2000